64 Sentai

64 Sentai

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This is incorporated on the 3’ end of the molecule synthesized, and is not template encoded. This overhanging A residue may help in cloning the products of PCR, but it depends on the strategy used. A number of polymerases are available from other Thermus species. These include Tfl and Tth enzymes from Thermus flavus and Thermus thermophilus respectively. These generally do not have 3’-5’ proof-reading activity. Polymerases are also available from other genera of bacteria (including archaebacteria), and many of these enzymes have 3’-5’ proof-reading activity (which also means they do not usually add terminal nucleotides that are not template directed).

G. single hairs or traces of body fluids, such as blood and semen) leads to applications in forensic work. A number of polymorphic regions have been used as targets for amplification, including: the D loop of mitochondrial DNA (mitochondrial DNA also has the advantage of being present at a high copy number per cell), which is variable between individuals 37 38 P OLYMER ASE CHAIN RE AC TION (much more so than the rest of the mitochondrial genome); the tandemly repeated minisatellites (also known as variable numbers of tandem repeats (VNTRs)) and microsatellites used in conventional genetic fingerprinting; and human leucocyte antigen (HLA) sequences.

3. DNA damage and polymerase error. Heterogeneity can also arise from damage to DNA before amplification, especially if the sample has not been carefully preserved. Therefore, this is particularly likely to be a problem with archaeological and forensic material. Damage includes oxidation or deamination of bases, and chemical cross-linking (within or between strands). As well as slowing down the polymerase (possibly resulting in the preferential amplification of undamaged, and perhaps contaminating, DNA), these changes may lead to the incorporation of incorrect bases during amplification.

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